Startup investments totaled $9.47 billion in the first three months of the year, up from $6.01 billion in the first quarter of 2013. It was the highest since the second quarter of 2001, when investments reached $11.5 billion.
There were 951 deals completed in the quarter, up from 916 in the same period a year ago.
Software companies received the most money - $4 billion. Biotech was a distant second with $1.06 billion. The last time the software sector received this much money was in the fourth quarter of 2000, right as the dot-com bubble was about to burst.
The sharp increase in venture funding in the first three months of the year comes amid a cooling of investor sentiment toward publicly traded technology stocks. Since March, shares of companies such as Netflix, Twitter and Facebook have sagged. With some technology stocks down as much as 40 percent, as in Twitter's case, the sharp decline is raising questions about whether the downturn is temporary or a sign that another bubble is about to pop.
That said, one reason for the high level of funding activity may be that VCs are investing in maturing companies. Later-stage deals are bigger than early-stage investments because they help startups expand rather than get off the ground.
Online storage startup Dropbox snagged the quarter's top deal with $325 million. It was the San Francisco company's fourth round of financing. Vacation rentals site Airbnb and mobile messaging service TangoMe tied for the No. 2 spot with $200 million each. For Airbnb, it was the seventh round of financing, while it was TangoMe's fourth.
The MoneyTree study was conducted by PricewaterhouseCoopers and the National Venture Capital Association, based on data from Thomson Reuters.